Dragon blood resin ameliorates steroid-induced osteonecrosis of femoral head through osteoclastic pathways

Objective: Dragon’s Blood resin (DBR) is a traditional medicinal substance renowned for its diverse pharmacological effects, which consists of potent anti-inflammatory, antioxidant and angiogenic properties. This study aimed to elucidate therapeutic mechanism in alleviating steroid-induced osteonecrosis the femoral head (SIONFH). Methods: Techniques such as SPR LC-MS were employed identify analyze target proteins DBR bone marrow macrophages (BMMs). In vitro , BMMs treated with RANKL DBR, TRAcP staining actin belt utilized assess osteoclast activity. The inhibitory effects underlying mechanisms on osteoclastogenesis reactive oxygen species (ROS) generation determined using real-time PCR, western blotting immunofluorescence staining. An vivo SIONFH rat model was set up curative impacts micro-CT scanning pathological Results: Bioinformatic tools revealed pivotal role differentiation SIONFH. Proteomic analysis identified 164 binding BMMs. assessments demonstrated that hindered by modulating expression specific genes proteins, along including TRX1 Glutathione Reductase. Notably, effectively inhibited crucial phosphorylation JNK nuclear localization p65 within TRAF6/JNK NFκB signaling pathways. experiments further confirmed mitigated onset rats curbing ROS activities. Conclusion: These findings underscore potential an effective administration early-stage SIONFH, shedding light influence ROS-mediated osteoclastic